Journal: Nucleic Acids Research

Article Title: Concentration-dependent exchange accelerates turnover of proteins bound to double-stranded DNA

doi: 10.1093/nar/gkq1140

Figure Lengend Snippet: Schematic of instrument and sample cell. ( a ) DNA is tethered between the wall of a square glass capillary tube and a paramagnetic bead. Force is applied using a permanent magnet to extend the DNA in the focal plane of a fluorescence microscope (force is to the left in schematic). Images are acquired using an EMCCD camera (see ‘Materials and Methods’ section for details). ( b ) Schematic of sample cell assembled from glass capillaries. Force is out of the page normal to the facing surface of the cell.

Article Snippet: Experiments were performed in 1 mm square glass capillary tubes (VitroCells, VitroCom, Mountain Lakes, NJ, USA) with a total volume in the experimental region of 10–20 μl.

Techniques: Fluorescence, Microscopy

Journal: Nucleic Acids Research

Article Title: A large field CCD system for quantitative imaging of microarrays

doi: 10.1093/nar/gkl160

Figure Lengend Snippet: Detection sensitivity. ( a ) Glycerol-PBS solutions containing known concentrations of Cy3 and Cy5 were loaded into rectangular fused silica capillaries, placed on a chromium-coated microscope slide, surrounded by glycerol and a glass cover slip applied. This mounting closely matched the indices of refraction of capillaries (fused silica ∼1.459, glycerol ∼1.473) so that very little light scatter was generated at the capillary surfaces. Five capillaries were mounted in an image field, each containing a mixture of Cy3 and Cy5 at concentrations of 0, 1, 10, 100 and 1000 molecules/µm 2 , and Cy3 and Cy5 images acquired. Concentrations of stock solutions were determined using absorbance measurements and known extinction coefficients. ( b ) Fluorescence intensities Cy3 (red curve) and Cy5 (black curve) increased linearly with fluorochrome density. The inset shows the portion of the curves between 0 and 10 fluorochromes/µm 2 , indicting the ability to detect concentrations below 1 fluorochrome/µm 2 .

Article Snippet: Solutions containing various concentrations of Cy3 and Cy5 in the glycerol/10% PBS medium we use for slide mounting were loaded into rectangular fused silica capillaries of interior dimensions 20 μm × 200 μm (Vitrotubes #5002S-050; VitroCom, Mountain Lakes NJ), and the capillaries were mounted on a chromium array substrate, as shown in .

Techniques: Microscopy, Generated, Fluorescence